Histochemical study on thiamine.

The authors have applied the thiochrome method for determining thiamine to histochemical investigations and could observe the localization of thiamine in cells and tissues (1-6). Independently of our study, v. Muralt (7) had already reported a investigation on histological pictures of thiamine in peri pheral nerves by the thiochrome method, which was however unavailable to the authors at that time. According to the retest of v. Muralt's method by the authors, preparations of peripheral nerves which can be observed comparatively well, were attained, but failed in case of organs containing much reducing substances such as the liver and kidneys. This paper deals with the procedure used at present by the authors and some histological pictures of main organs obtained by this method.